Work Station |
Title of Course |
Objectives |
1. Moderators: Čavlović K, Gelo N, Rakoš Justament R |
Semen analysis |
Participants will learn how to assess the following:
- volume,
- coagulation,
- liquefaction,
- viscosity,
- sperm count,
- sperm concentration (chambers: Makler, Neuebauer hematocytometer, Cell Vision, Cell-VU, Microcell),
- sperm motility,
- differences between manual and CASA semen analysis,
- preparation of a seminal smear for morphology
|
2. Moderators: Tramišak Milaković T, Dundović M |
Sperm Morphology |
Participants will learn how to evaluate sperm morphology using Strict criteria:
- prestained slides
- Giemsa dye
|
3. Moderators: Ljiljak D, Matković K |
HBA test |
Preselection test for PICSI:
|
4. Moderators: Panić Horvat L, Zovkić S |
Vitality test and Hypoosmotic sweling test |
Participants will learn how to make:
- Vitality test (Eosin-Nigrosin stain): how to distinguish dead and live sperm in a poor motility semen sample.
- HOS test evaluates sperm membrane integrity and is used to select live sperm for ICSI in a population of non-motile sperm
|
5. Moderators: Vujisić S, Šogorić Ševo S, Matić I |
Sperm DNA fragmentation |
Participants will learn how to measure sperm DNA damage using HALO test. They will learn about other techniques for the same purpose. |
6. Moderators: Hafner D, Špoljarić N, Čerina M |
Sperm Preparation Methods |
Partcipants will be acquainted on shematic slides about methods of sperm preparation.
- ejaculated sperm preparation for AIH,
- ejaculated sperm preparation for IVF,
- ejaculated sperm preparation for ICSI,
- sperm preparation after retrograde ejaculation,
- sperm preparation for ICSI after TESE/PESA/MESA
Basic sperm preparation techiques: swim-up technique, density gradient technique, MACS Sperm selection techniques: PVP, PICSI, IMSI |
7. Moderators: Stanić P, Dobec D, Štimac D |
Semen Cryopreservation for Ejaculated and Testicular sperm |
Participants will learn tips and tricks in cryopreservation of fresh ejaculated semen and testicular sperm. |
8. Moderators: Baričević M, Vujisić S |
Controlling for quality in the andrology laboratory |
Participants will learn about QC and essential elements for good laboratory practice. |
Croatian Society of Clinical Embryologists |
Sperm analysis report |
Participants will learn what are minimal proposals for a good quality sperm analysis report.
- Revision of last consensus documents
|